Drawing II Glass Tips

[sipa]

Does anyone here draw their own glass tips for use in II

If you do, what puller do you use and what was the cost. There are a few on the auction sites and was contemplating having a go.

 

[Gilberdyke John]

Does anyone here draw their own glass tips for use in II

If you do, what puller do you use and what was the cost. There are a few on the auction sites and was contemplating having a go.

I have in the past, but not beekeeping related, drawn glass tube and rod out simply using a bunsen burner. If you draw the softened /molten section out by hand and with practice wouldn't that achieve what you are after?

 

[sipa]

I have in the past, but not beekeeping related, drawn glass tube and rod out simply using a bunsen burner. If you draw the softened /molten section out by hand and with practice wouldn't that achieve what you are after?

Accuracy and consistency is key, so I wouldn't be confident of doing it in the way you suggest myself, but others might.

 

[For want of a better word]

http://www.dave-cushman.net/bee/makingtips.html

Having trained as an Instrument Maker in one of my reincarnations I have made my own... at 25 quid + each from Abelo et al they are not cheap.
The drop machine is the way that a consistent tube diameter can easily be achieved.
Somewhere on the net is a demonstration of how to build and operate.
If you follow the kinks on Dave Cushman's site ( now edited by Rodger Patterson) you will find lots of useful info.

Yeghes da

 

[B+.]

Accuracy and consistency is key, so I wouldn't be confident of doing it in the way you suggest myself, but others might.

It depends how ambitious you want to be: Dr Peter Schley sells one (which I think most people are familiar with), but, you could go all the way up to the Sutter models which could cost you an arm and a leg (http://www.sutter.com/MICROPIPETTE/p-2000.html)

 

[megadyptes]

Does anyone here draw their own glass tips for use in II

If you do, what puller do you use and what was the cost. There are a few on the auction sites and was contemplating having a go.

In the dim and distant past, I used to make a lot of micro-electrodes using glass pullers. The two standard bits of kit you'd find in all the labs were the following:

http://www.zeitz-puller.com/
http://products.narishige-group.com...uct=PC-10&category=injection&language=english

I dare say they are overkill for what you're trying to do, but they gave consistent micro-electrodes over the course of a few years with minimal maintenance. You could probably find them on the auction sites at a fraction of their new-build costs, but i guess they're still not going to be that cheap.

 

[megadyptes]

It depends how ambitious you want to be: Dr Peter Schley sells one (which I think most people are familiar with), but, you could go all the way up to the Sutter models which could cost you an arm and a leg (http://www.sutter.com/MICROPIPETTE/p-2000.html)

Dang, that's another one you'd see around the labs quite frequently - i used to play with that one as well... happy days!

 

[B+.]

Dang, that's another one you'd see around the labs quite frequently - i used to play with that one as well... happy days!

Perhaps a bit OTT for II though. ;-)

 

[megadyptes]

Perhaps a bit OTT for II though. ;-)

Yep, wouldn't argue with that sentiment, but nice to reminisce about the good olde days.

 

[sipa]

It depends how ambitious you want to be: Dr Peter Schley sells one(http://www.sutter.com/MICROPIPETTE/p-2000.html)

Yes, I know the Schley model exists and that would be my fallback purchase.

There are others kicking around for 2-400 USD that appear to offer more versatility.

 

[thenovice]

I used the Sutter and Narashige models for work with zebrafish. the Sutter is a fantastic piece of equipment if you need to transplant cells or inject DNA/RNA constructs into single cells but for II it seems overkill. Narashige models are less accurate and reproducible but for II I assume they will do just fine. you could also pull yourself over bunsen and then break of tip at required diameter.

 

[B+.]

you could also pull yourself over bunsen and then break of tip at required diameter.

Not so reproducible though, but, it he wanted to play around with tip profiles it could be an option

 

[megadyptes]

Another option dragged back from the aged grey matter...
http://www.wpi-europe.com/products/glass/tip-pre-pulled-glass-capillaries.aspx

 

[sipa]

Thanks for all the suggestions. Will let you know what I end up with.

 

[dano41]

Have you seen this home made one that works with a battery charger the tips this system produces are very consistent and it is relatively easy to make.https://www.youtube.com/watch?v=0_ii8fS4Be0

 

[oliver90owner]

Simple enough to draw your own, I would have thought. A microscope to check the size would be a simple expediant. Depends a lot on how many one might wish to reject, before getting it right?